Composite

Part:BBa_K2066034

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


mCherry + Spinach, J23107 + B0034

This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. Spinach and mCHerry allow characterization of gene expression at the RNA and Protein level, respectively. The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB and Spinach aptamer sequence are taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1052
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal SpeI site found at 1052
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1052
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None