Composite
Part:BBa_K2066034
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-14)
mCherry + Spinach, J23107 + B0034
This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016. Spinach and mCHerry allow characterization of gene expression at the RNA and Protein level, respectively. The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB and Spinach aptamer sequence are taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1052
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70
Illegal SpeI site found at 1052 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1052
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1052
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |